GbR offers Predictive Functional Profiling (PFP) for your 16S data. This approach combines taxonomic information from 16S rRNA gene data with functional information (e.g., metabolic pathways) derived from closely related taxa with sequenced genomes. PFP is a powerful and cost-efficient alternative to shotgun metagenomics for well characterized microbial samples, e.g., from the human intestine. It provides new insights into the functional aspects of uncultivated microbial communities for which only 16S rRNA gene data are currently available.