16S rRNA amplicon metagenomics
Efficient characterization of microbial communities
omics2view.consulting GbR and partners offer fast, dependable, and cost-effective 16S rRNA amplicon metagenomic analysis.
We recommend the following primer systems targeting different variable regions for sequencing on the Illumina MiSeq system (EMP = Earth Microbiome Project; HMP = Human Microbiome Project):
| Primer pair | Sequence | Region | Size (bp) | Reference |
|---|---|---|---|---|
| 515f
806r |
GTGCCAGCMGCCGCGGTAA
GGACTACHVGGGTWTCTAAT |
V4 | ~300 | EMP |
| 27F_hmp
534R_hmp |
AGAGTTTGATCCTGGCTCAG
ATTACCGCGGCTGCTGG |
V1-V3 | ~510 | HMP |
| 341F_ill
802R_ill |
CCTACGGGNGGCWGCAG
GACTACHVGGGTATCTAATCC |
V3-V4 | ~460 | Illumina |
| 341F
806R |
ACTCCTACGGGAGGCAGCAG
GGACTACHVGGGTWTCTAAT |
V3-V4 | ~460 | |
Of course, we can employ any customized primer set upon request. We will also help you design optimal primers for targeting specific organisms.
Important note: For library preparation, the above primer sequences need to be combined with specific sequencing adapters. Sequencing will not work with amplicons produced with ordinary primers. Kindly provide us with DNA isolated from your microbial communities. Or simply send us your clinical or environmental samples for DNA isolation.
